水素水が急性腹膜炎ラットモデルに与える影響の検討
Three distinct rat models of acute peritonitis were established using intraperitoneal lipopolysaccharide (LPS), fecal injection, or cecal ligation and puncture (CLP). Male Sprague-Dawley rats received hydrogen-rich water (HRW, 3 ml per rat) or saline by oral gavage for 7 days before and 3 days after model induction. Across all three models, HRW administration significantly reduced circulating white blood cell counts, plasma endotoxin levels, and pro-inflammatory cytokines IL-6 and TNF-α. In visceral peritoneal tissue, HRW elevated glutathione (GSH) activity while decreasing myeloperoxidase (MPO) and malondialdehyde (MDA) levels. Immunohistochemical analysis revealed suppressed NF-κB expression in peritoneal tissue, and histopathological examination confirmed reduced tissue damage. These findings indicate that HRW attenuates acute peritonitis severity through combined anti-inflammatory, antioxidant, and antibacterial mechanisms.
HRW suppresses NF-κB expression in peritoneal tissue, leading to reduced pro-inflammatory cytokines (IL-6, TNF-α) and oxidative stress markers (MDA, MPO), while enhancing the antioxidant glutathione (GSH), collectively mitigating peritoneal inflammation.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/24793096