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Molecular hydrogen regulates gene expression by modifying the free radical chain reaction-dependent generation of oxidized phospholipid mediators.

水素ガスはフリーラジカル連鎖反応依存的な酸化リン脂質メディエーターの生成を修飾することで遺伝子発現を調節する

in vitro study in vitro positive 1%

Abstract

This study investigated the mechanism by which H2 controls gene expression. In a cell-free chemical system, approximately 1% H2 gas (v/v) inhibited the autoxidation of linoleic acid driven by free radical chain reactions. When the major phospholipid PAPC was autoxidized in the presence or absence of H2, the resulting profile of oxidized phospholipid species differed between conditions. Cultured cells exposed to H2-modified oxidized phospholipid species showed attenuated Ca²⁺ signaling, and comprehensive microarray analysis revealed broad changes in gene expression. Within intact cultured cells, H2 suppressed free radical-mediated lipid peroxidation and normalized elevated intracellular Ca²⁺ levels, thereby modulating Ca²⁺-dependent transcriptional programs. These findings suggest that H2 influences gene expression through the Ca²⁺ signaling pathway by altering the spectrum of oxidized phospholipid mediators generated during free radical chain reactions.

Mechanism

H2 suppresses free radical chain reactions, altering the oxidized phospholipid (PAPC) species profile. This modification normalizes intracellular Ca²⁺ signaling, which in turn regulates Ca²⁺-dependent gene expression across multiple pathways.

Bibliographic

Authors
Iuchi K, Imoto A, Kamimura N, Nishimaki K, Ichimiya H, Yokota T, et al.
Journal
Sci Rep
Year
2016 (2016-01-07)
PMID
26739257
DOI
10.1038/srep18971
PMC
PMC4704061

Tags

Mechanism:抗酸化酵素 ヒドロキシルラジカル消去 炎症抑制 脂質過酸化 ミトコンドリア 酸化ストレス 活性酸素種

Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:

Cite as: H2 Papers — PMID 26739257. https://h2-papers.org/en/papers/26739257
Source: PubMed PMID 26739257