マクロファージにおけるパラコート誘発性ROS・TNF-α産生に対する分子状水素とSAHAの効果
This in vitro study examined how molecular hydrogen (H2) and the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) affect paraquat (PQ)-stimulated reactive oxygen species (ROS) and tumor necrosis factor-alpha (TNF-α) in RAW264.7 macrophages. A PQ concentration of 0.1 mM was identified as producing significant, non-cytotoxic stimulation of ROS and TNF-α. H2 suppressed PQ-induced ROS generation and reduced TNF-α levels during the early phase (1 and 2 hours), while SAHA was more effective at attenuating TNF-α during the late phase (8 hours). Combining H2 with SAHA produced greater ROS reduction than either agent alone, particularly at 2 and 8 hours, indicating complementary and potentially synergistic actions between the two compounds in macrophage inflammatory responses.
H2 scavenges ROS and suppresses early-phase TNF-α production in macrophages, while SAHA inhibits histone deacetylase activity to attenuate late-phase TNF-α. Their combination yields additive or synergistic suppression of paraquat-induced oxidative and inflammatory responses.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/27624060