水素水がシメジ（Hypsizygus marmoreus）の抗酸化系・菌糸再生・子実体形成に及ぼす酸化還元調節作用
This study examined the role of hydrogen-rich water (HRW) in the redox regulation of the industrially cultivated mushroom Hypsizygus marmoreus. Mechanically damaged mycelia were exposed to HRW, resulting in accelerated regeneration and significantly greater fruiting body yields compared with untreated controls. Antioxidant enzyme activities and antioxidant compound levels rose markedly following HRW exposure, while reactive oxygen species (ROS) and malondialdehyde (MDA) concentrations declined during the mycelial regeneration phase. Gene expression analyses revealed upregulation of components in ROS, calcium, MAPK, and oxylipin signaling pathways. Laccase and manganese peroxidase activities, along with mycelial biomass, were also elevated, suggesting that HRW may accelerate substrate degradation and thereby increase carbon source availability for fruiting body development.
HRW upregulates genes in ROS, calcium, MAPK, and oxylipin signaling pathways, enhances antioxidant enzyme activities, and reduces ROS and MDA levels, collectively promoting mycelial regeneration and fruiting body development in H. marmoreus.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/29665957