水素リッチウォーターによるゼブラフィッシュ鱗モデルでのグルコルチコイド誘発性骨粗鬆症における破骨細胞活性化抑制
Using zebrafish scales as a secondary osteoporosis model, prednisolone (PN) administration elevated osteoclast activity while reducing osteoblast function, as confirmed by bone enzymatic assays. Hydrogen-rich water (HRW) was evaluated as an antioxidant intervention. Both biochemical and histochemical tartrate-resistant acid phosphatase (TRAP) assays demonstrated that HRW effectively blocked osteoclast activation and attenuated bone loss in PN-exposed scales. However, HRW did not restore osteoblast activity, as measured by alkaline phosphatase, nor did it facilitate repair of resorption lacunae induced by PN. These findings indicate that HRW exerts a selective inhibitory effect on mature osteoclasts without influencing osteoblast function, suggesting a targeted antioxidant mechanism relevant to bone resorption.
HRW is proposed to scavenge reactive oxygen species, thereby suppressing osteoclast activation signals and reducing bone resorption, while leaving osteoblast activity unaffected in the glucocorticoid-exposed zebrafish scale model.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/36829904