体外循環回路における分子状水素の抗炎症・抗酸化効果：インビトロパイロット研究
Extracorporeal circulation (ECC) modalities such as ECMO and hemodialysis are associated with chronic inflammation and oxidative stress, partly attributable to the circuits themselves. This in vitro pilot study used miniature ECMO circuits primed with heparinized blood from seven healthy adult donors to evaluate the effects of H2 gas on pro-inflammatory conditions. Circuits were assigned to control, LPS-stimulated, or LPS-stimulated plus H2 gas conditions. After 6 hours, biomarkers including MCP-1, MPO, MDA-a, TRX1, and IL-6 were measured. Circulation alone induced blood trauma and inflammatory responses, while LPS markedly amplified these effects. Notably, the H2-treated circuit showed a tendency toward lower concentrations of the measured biomarkers compared to the LPS-only circuit, suggesting a potential antioxidant and anti-inflammatory role for molecular hydrogen in ECC settings. The authors note that further studies are needed to optimize dosage, duration, and administration cycles.
Molecular hydrogen is proposed to selectively scavenge reactive oxygen species, thereby attenuating LPS-induced elevations in inflammatory cytokines (IL-6, MCP-1) and oxidative stress markers (MPO, MDA-a) within extracorporeal circulation circuits.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/39200347