日本語View as Markdown

The Rieske iron-sulfur protein is a primary target of molecular hydrogen.

リスケ鉄硫黄タンパク質は分子状水素の主要な標的である

in vitro study mixed routes positive

Abstract

The biomedical mechanisms of molecular hydrogen (H₂) have long been attributed primarily to hydroxyl radical scavenging, with H₂ generally considered biologically inert. This study challenges that view by identifying the Rieske iron-sulfur protein (RISP) as a direct molecular target of H₂. Mitochondrial unfolded protein response (UPRmt) was observed both in cultured cells exposed to H₂ and in mouse liver following hydrogen-rich water administration. In mouse liver homogenates, H₂ reduced electron transport chain complex III activity to 78.5% within 2 minutes. Drawing on the evolutionary relationship between RISP and hydrogenases, the authors found that H₂ activates mitochondrial Lon peptidase 1 (LONP1), leading to RISP degradation within 1 hour in cultured cells. The resulting loss of RISP and subsequent UPRmt induction provide a mechanistic framework for the pleiotropic and paradoxical biological effects of H₂, establishing it as an active signaling molecule rather than a passive antioxidant.

Mechanism

H₂ activates mitochondrial Lon peptidase 1 (LONP1), promoting degradation of the Rieske iron-sulfur protein (RISP) within 1 hour. This suppresses electron transport chain complex III activity and triggers the mitochondrial unfolded protein response (UPRmt), positioning H₂ as a biologically active signaling molecule.

Bibliographic

Authors
Negishi S, Ito M, Hasegawa T, Otake H, Ohkawara B, Masuda A, et al.
Journal
Redox Biol
Year
2025
PMID
41330217
DOI
10.1016/j.redox.2025.103952
PMC
PMC12719095

Tags

Delivery:水素水経口投与 Mechanism:ヒドロキシルラジカル消去 炎症抑制 ミトコンドリア 酸化ストレス 活性酸素種

Delivery context

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

Safety notes

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:

Cite as: H2 Papers — PMID 41330217. https://h2-papers.org/en/papers/41330217
Source: PubMed PMID 41330217