重水素同位体を用いたラットにおける水素ガス代謝の定量的評価
This study investigated the quantitative metabolic parameters of molecular hydrogen in vivo using intraperitoneally administered deuterium gas as a tracer, with deuterium enrichment measured in the body water pool. Under physiological conditions in rats, approximately 10% of the administered dose underwent oxidation, providing evidence of antioxidant activity. Neither hypoxic conditions nor endotoxin administration altered deuterium oxidation, whereas pure oxygen inhalation led to a reduction in oxidation. In parallel in vitro experiments using bovine heart submitochondrial particles, hydrogen significantly suppressed superoxide generation at Complex I of the mitochondrial respiratory chain. The authors discuss iron-sulfur clusters as potential mediators of reactive oxygen species production and their interaction with dihydrogen as a plausible mechanistic basis for the observed effects.
Hydrogen is proposed to interact with iron-sulfur clusters in mitochondrial respiratory chain Complex I, thereby reducing superoxide generation. In vivo, approximately 10% of administered hydrogen undergoes oxidation under physiological conditions, consistent with direct antioxidant activity.
This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/26103048