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Protective effects of molecular hydrogen on steroid-induced osteonecrosis in rabbits via reducing oxidative stress and apoptosis.

分子状水素がウサギのステロイド誘発性骨壊死に対して示す酸化ストレス・アポトーシス抑制を介した保護効果

animal study injection / infusion positive

Abstract

This animal study examined whether molecular hydrogen could reduce the occurrence of steroid-induced osteonecrosis (ON) in rabbits. Sixty rabbits were randomly allocated to a model group or a hydrogen group; the latter received intraperitoneal hydrogen injections at 10 ml/kg daily for seven days. Histopathological analysis revealed that ON incidence was markedly lower in the hydrogen group (28.6%) compared with the model group (68.0%). Immunohistochemical staining for 8-OHdG and MDA, along with TUNEL assays, demonstrated that oxidative injury, vascular injury, and apoptosis were all reduced in hydrogen-treated animals. Plasma cholesterol and triglyceride levels did not differ significantly between groups. These findings indicate that hydrogen administration suppresses oxidative and vascular damage as well as apoptotic cell death, thereby lowering the incidence of steroid-induced osteonecrosis.

Mechanism

Molecular hydrogen acts as a selective antioxidant, reducing oxidative damage markers (8-OHdG, MDA) and suppressing vascular injury and apoptotic cell death, collectively lowering the incidence of steroid-induced osteonecrosis in vivo.

Bibliographic

Authors
Li J, Ge Z, Fan LY, Wang K
Journal
BMC Musculoskelet Disord
Year
2017 (2017-02-02)
PMID
28148301
DOI
10.1186/s12891-017-1431-6
PMC
PMC5288900

Tags

Delivery:点滴投与 Mechanism:アポトーシス抑制 グルタチオン ヒドロキシルラジカル消去 脂質過酸化 酸化ストレス 活性酸素種

Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:

Cite as: H2 Papers — PMID 28148301. https://h2-papers.org/en/papers/28148301
Source: PubMed PMID 28148301