マイコトキシン汚染飼料を給与した雌豚仔における小腸の形態的・分子的応答に対するラクツロースおよび水素豊富水の影響
Twenty-four female piglets were divided into four groups: negative control, mycotoxin-contaminated (MC) feed alone, MC feed with lactulose (LAC, 500 mg/kg BW twice daily), and MC feed with hydrogen-rich water (HRW, 10 mL/kg BW twice daily). After 25 days, MC-exposed piglets showed elevated serum diamine oxidase activity, D-lactic acid, and endotoxin levels, along with reduced villus height and villus-to-crypt ratios, increased apoptosis, altered tight junction gene expression, and disrupted claudin-3 (CLDN3) protein distribution in the small intestinal mucosa. Both HRW and LAC administration significantly counteracted these mycotoxin-induced changes, preserving intestinal morphology, maintaining tight junction integrity, and restoring CLDN3 expression and localization. These findings indicate that oral HRW supplementation can protect small intestinal barrier function in piglets consuming mycotoxin-contaminated diets.
HRW is thought to suppress mycotoxin-induced apoptosis and normalize tight junction gene expression along with CLDN3 protein distribution, thereby preserving the intestinal epithelial barrier against toxin-driven permeability increases.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/30805184