水素水処理がアルファルファのUVB誘発酸化傷害を軽減する：（イソ）フラボノイド代謝と抗酸化防御機構の関与
In alfalfa (Medicago sativa) seedlings exposed to ultraviolet-B (UVB) irradiation, endogenous H2 production was elevated, and pretreatment with hydrogen-rich water (HRW) reproduced this response. HRW pretreatment substantially reduced lipid peroxidation and reactive oxygen species (ROS) accumulation, and alleviated growth inhibition caused by UVB stress. Metabolic profiling via UPLC-MS identified 40 (iso)flavonoid compounds in UVB-exposed plants, of which 22 were upregulated by HRW, with isoflavone and flavanone subfamilies showing the most pronounced increases. Specific compounds elevated included daidzein, formononetin glucoside-malonate, naringenin, and garbanzol, among others. In vitro assays confirmed that the HRW-modulated (iso)flavonoid profile exhibited enhanced ROS-scavenging and antioxidant capacity. Transcriptional analysis revealed that HRW further amplified UVB-induced upregulation of (iso)flavonoid biosynthetic genes, while activities and transcript levels of key antioxidant enzymes were also increased. These findings collectively indicate that HRW-conferred UVB tolerance in alfalfa is partially mediated through coordinated regulation of (iso)flavonoid metabolism and antioxidant defense systems.
HRW amplifies transcription of (iso)flavonoid biosynthetic genes, increasing accumulation of isoflavone and flavanone subclasses that scavenge ROS, while simultaneously inducing antioxidant enzyme activity, thereby reducing UVB-induced oxidative stress in alfalfa.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/32480752