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Effects of Inhalation of Hydrogen Gas on Allergic Conjunctivitis Model Mice.

水素ガス吸入がアレルギー性結膜炎モデルマウスに与える影響

animal study inhalation positive

Abstract

Using an ovalbumin-sensitized mouse model of allergic conjunctivitis, this study examined whether inhaled hydrogen gas could modify allergic ocular responses. Mice received intraperitoneal ovalbumin sensitization followed by daily ocular instillation of the antigen. After antigen challenge, animals in the hydrogen group were exposed to hydrogen gas. Two primary outcomes were assessed: eye-scratching frequency as a surrogate for ocular pruritus, and eosinophil counts in tear fluid. Hydrogen gas exposure significantly reduced antigen-induced scratching behavior and attenuated the rise in tear eosinophil numbers. The authors propose that reactive oxygen species play a central role in both pruritic signaling and eosinophil recruitment, and that hydrogen gas scavenges these species to suppress allergic conjunctival inflammation.

Mechanism

Hydrogen gas is proposed to scavenge reactive oxygen species, thereby suppressing pruritic signaling pathways and reducing eosinophil migration into conjunctival tissue.

Bibliographic

Authors
Sakata H, Kanzaki S, Kai H, Morii H, Haraoka N, Takayama S, et al.
Journal
J Ocul Pharmacol Ther
Year
2026
PMID
42101321
DOI
10.1177/10807683261415800

Tags

Disease:網膜疾患 Delivery:吸入投与 Mechanism:ヒドロキシルラジカル消去 免疫調節 炎症抑制 酸化ストレス 活性酸素種

Delivery context

For inhalation applications of molecular hydrogen, the lower flammability limit (LFL) deserves careful handling. The classical 4% figure applies to closed-system mixtures; the practical inhalation-environment threshold is 10%. Even pure-hydrogen output (the UFL 75% paradox) passes through the flammable range at the air–gas boundary. High-concentration (66% / 100%) inhalers are documented in the Japanese Consumer Affairs Agency accident-information database and are not recommended.

Safety notes

For inhalation applications of molecular hydrogen, the lower flammability limit (LFL) deserves careful handling. The classical 4% figure applies to closed-system mixtures; the practical inhalation-environment threshold is 10%. Even pure-hydrogen output (the UFL 75% paradox) passes through the flammable range at the air–gas boundary. High-concentration (66% / 100%) inhalers are documented in the Japanese Consumer Affairs Agency accident-information database and are not recommended.

See also:

Other papers on the same disease / condition

Cite as: H2 Papers — PMID 42101321. https://h2-papers.org/en/papers/42101321
Source: PubMed PMID 42101321