水素富化水浴を用いた心臓移植グラフト保存の新手法
Using a rat heterotopic cardiac transplantation model, this study examined the efficacy of cold organ storage in a hydrogen-rich water bath generated by an electrolyzer. Both syngeneic grafts from elderly donors (60–70-week-old Lewis rats) and allografts from adult donors (12-week-old Brown Norway rats) underwent prolonged cold preservation in Celsior solution within bags immersed in the hydrogen-saturated bath. Control grafts showed marked elevation of serum troponin I and creatine phosphokinase at 3 hours post-reperfusion, accompanied by neutrophil infiltration and upregulation of pro-inflammatory cytokine and chemokine mRNAs. Grafts preserved in the hydrogen-rich bath demonstrated significantly reduced myocardial injury and inflammatory responses, along with decreased mitochondrial damage and higher adenosine triphosphate content. These findings indicate that hydrogen delivery via a water bath during cold storage effectively mitigates ischemia-reperfusion injury in cardiac grafts.
Hydrogen exerts antioxidant, anti-inflammatory, and anti-apoptotic effects that suppress reactive oxygen species generated during cold ischemia-reperfusion, thereby reducing neutrophil infiltration, pro-inflammatory cytokine expression, and mitochondrial damage while preserving ATP content in cardiac grafts.
Hydrogen bathing has reports of localized effects, but for systemic hydrogen intake the most efficient route is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/23273745