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Transplantation of High Hydrogen-Producing Microbiota Leads to Generation of Large Amounts of Colonic Hydrogen in Recipient Rats Fed High Amylose Maize Starch.

高アミロースとうもろこしデンプン給餌ラットにおける高水素産生腸内細菌叢の移植による門脈水素濃度上昇と酸化ストレス軽減

animal study animal model positive

Abstract

This animal study investigated whether elevated portal vein H2 concentrations in rats consuming a 20% high amylose maize starch (HAS) diet could reduce oxidative stress, and whether transplanting colonic microbiota from high-H2-generating (HG) rats could convert low-H2-generating (LG) rats. In experiment 1, following 10 days of HAS feeding and hepatic ischemia-reperfusion surgery, rats with higher portal H2 concentrations showed significantly lower plasma alanine aminotransferase activity and hepatic oxidized glutathione levels. In experiment 2, oral inoculation of HG-derived microbiota into LG rats on days 3 and 4 resulted in significantly elevated portal H2 concentrations by day 13 compared with saline-treated controls. Specific bacterial genera showed positive or negative correlations with portal H2 levels. These findings indicate that microbiota transplantation can stably shift H2 production capacity and that the resulting H2 elevation is associated with reduced oxidative stress markers.

Mechanism

H2 generated by colonic bacterial fermentation reaches the liver via the portal vein, where it is associated with reductions in oxidized glutathione and alanine aminotransferase activity, suggesting attenuation of hepatic oxidative stress.

Bibliographic

Authors
Nishimura N, Tanabe H, Komori E, Sasaki Y, Inoue R, Yamamoto T
Journal
Nutrients
Year
2018 (2018-01-29)
PMID
29382125
DOI
10.3390/nu10020144
PMC
PMC5852720

Tags

Disease:虚血再灌流障害 肝疾患 Mechanism:抗酸化酵素 グルタチオン ヒドロキシルラジカル消去 炎症抑制 酸化ストレス

Delivery context

This study is at the animal-experiment stage. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

Safety notes

This study is at the animal-experiment stage. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:

Other papers on the same disease / condition

Cite as: H2 Papers — PMID 29382125. https://h2-papers.org/en/papers/29382125
Source: PubMed PMID 29382125