水素吸蔵合金カニスターを用いた水素溶解臓器保存液によるミニブタ虚血腎移植モデルでの機能改善
A practical approach using a hydrogen-absorbing alloy canister was developed to dissolve H2 gas into organ preservation solutions within 2–3 minutes. ETK solution pressurized at 0.06 MPa maintained dissolved H2 concentrations at or above 1.0 mg/L for 4 hours after opening. In a miniature pig kidney transplantation model involving 30 minutes of warm ischemia from circulatory arrest, donor kidneys flushed and stored in H2-containing ETK solution for 1 or 4 hours showed detectable urine production and confirmed blood flow in the transplanted organ at postoperative day 6. Kidneys stored in H2-free solution produced no urine and showed no detectable blood flow at the same time point. Perfusion rate during initial flushing was also faster with H2-containing solution. These findings indicate that this rapid H2 dissolution method may enable conversion of ischemia-damaged organs into viable grafts under practical clinical conditions.
H2 dissolved in the preservation solution is thought to scavenge reactive oxygen species generated during ischemia-reperfusion, thereby reducing oxidative damage to the donor kidney and facilitating functional recovery after transplantation.
Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).
See also:
https://h2-papers.org/en/papers/31574107