アルカノイルアスコルベートと白金ナノコロイドの併用による抗腫瘍効果および水素による加水分解産物アスコルビン酸の安定化
Using human esophageal carcinoma-derived KYSE70 cells, the combination of 6-O-palmitoyl ascorbate (Asc6Palm) and 2-nm platinum-poly(N-vinyl-pyrrolidone) colloid (PVP-Pt) suppressed cell viability more substantially than either agent alone, accompanied by cell shrinkage and fragmentation. No additive effect was observed with unmodified ascorbic acid or its 2-O-phospho derivative combined with PVP-Pt. The superior activity of Asc6Palm was attributed to a favorable balance of molecular hydrophilicity and lipophilicity (BMHL) facilitating intracellular uptake. Longer-chain or dipalmitoyl derivatives showed reduced activity, consistent with suboptimal BMHL. Ascorbic acid generated by esterolytic conversion of Asc6Palm was found to be appreciably stabilized in electrolytically produced hydrogen-rich water (dissolved hydrogen: 0.575 mg/L) as assessed by HPLC-coulometric electrochemical detection, whereas hydrogen-gas-bubbled water (0.427 mg/L), Mg-stick-derived hydrogen water (0.044 mg/L), and tap water provided little stabilization. These findings indicate that hydrogen-rich water can suppress oxidative degradation of ascorbic acid, potentially supporting its retention for effective antitumor activity.
Electrolytically generated hydrogen-rich water suppresses oxidative decomposition of ascorbic acid produced by esterolysis of Asc6Palm, thereby maintaining intracellular ascorbate levels that contribute to the antitumor effect in combination with PVP-Pt.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/33387361