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Protective effects of hydrogen-rich water against acute lead-induced hepatic, renal, and testicular toxicity in rats.

水素水が急性鉛曝露ラットの肝臓・腎臓・精巣毒性に及ぼす保護効果

animal study hydrogen-rich water positive

Abstract

Lead acetate (PbA) exposure causes multi-organ damage through oxidative stress, mitochondrial dysfunction, and inflammation. In this animal study, 30 male Wistar rats were allocated to five groups—control, PbA, EDTA, hydrogen-rich water (HRW), and HRW+EDTA—and assessed after one week for body weight, organ weights, serum biochemistry, histopathology, and oxidative stress markers (MDA, SOD, catalase) in liver, kidney, and testis. PbA induced approximately 13% body weight loss, roughly doubled serum creatinine and liver enzymes, elevated tissue MDA, and reduced antioxidant enzyme activity. HRW alone significantly lowered MDA and restored catalase activity in liver and testis. EDTA alone reduced hepatic MDA by approximately 30% but did not recover antioxidant enzyme activity. The combined HRW+EDTA regimen reduced MDA but yielded lower catalase activity than HRW alone, and SOD showed no consistent recovery across any group. These findings indicate that HRW confers statistically significant organ-protective effects against acute lead toxicity, with catalase restoration appearing to be a key contributing mechanism.

Mechanism

HRW is proposed to suppress mitochondrial reactive oxygen species production, thereby reducing lipid peroxidation (MDA) and restoring catalase activity in liver and testis, which collectively mitigates lead-induced oxidative organ damage.

Bibliographic

Authors
Kharazmi K, Saroughi M, Khoshniat MT, Nazari SE, Asadi M, Rostami A, et al.
Journal
Naunyn Schmiedebergs Arch Pharmacol
Year
2026 (2026-05-12)
PMID
42115396
DOI
10.1007/s00210-026-05422-6

Tags

Disease:重金属毒性 鉛中毒 Delivery:水素水経口投与 Mechanism:抗酸化酵素 炎症抑制 脂質過酸化 ミトコンドリア 酸化ストレス

Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:

Other papers on the same disease / condition

Cite as: H2 Papers — PMID 42115396. https://h2-papers.org/en/papers/42115396
Source: PubMed PMID 42115396