水素水がミミズにおけるニッケル誘発毒性（炎症・酸化ストレス・DNA損傷）を軽減し繭生産を改善する
This study examined the capacity of hydrogen-rich water (HRW) to counteract nickel chloride toxicity in an earthworm model over a 14-day exposure period at concentrations of 10, 200, and 500 mg nickel per kg soil. Compared with groups receiving untreated water, earthworms exposed to nickel in HRW showed markedly lower reactive oxygen species (ROS) levels, reduced expression of the DNA oxidation marker 8-hydroxy-2'-deoxyguanosine (8-OHdG), and diminished TNF-α-mediated inflammatory signaling. Histopathological assessment revealed less epithelial degeneration, epithelial necrosis, and muscle fiber necrosis in HRW-treated animals. Cocoon production, which declined under high nickel exposure, was better preserved in the HRW groups. These findings indicate that HRW can mitigate heavy-metal-induced oxidative and inflammatory injury in soil organisms, suggesting potential utility as an environmentally benign approach to managing heavy-metal contamination.
HRW scavenges reactive oxygen species generated by nickel exposure, thereby suppressing ROS accumulation, TNF-α-driven inflammatory responses, and 8-OHdG DNA oxidative damage, which collectively reduces histopathological tissue injury in earthworms.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/34482505