ATR-FTIRおよびLC-ESI-MS/MS分光法を用いたミミズにおける水素水の水銀毒性軽減効果の評価
This study examined mercury chloride toxicity in earthworms and whether hydrogen-rich water (HRW) could reduce its harmful effects, using ATR-FTIR and LC-MS/MS as analytical tools. Earthworms received injections of mercury chloride at four concentrations (5, 10, 20, and 40 µg/mL) prepared either in standard water or in HRW. FTIR spectral bands associated with proteins, lipids, and polysaccharides were more markedly diminished in the standard mercury group at 20 µg/mL compared with the corresponding HRW group, indicating partial biomolecular protection. LC-MS data from the highest dose groups revealed that the 8-Oxo-dG marker ion (m/z 283.1), indicative of oxidative DNA damage, was elevated to a greater extent in the standard mercury group than in the HRW group. These findings suggest that HRW possesses the capacity to partially counteract mercury-induced oxidative injury, though extended exposure durations may be required to fully characterize the protective magnitude. ATR-FTIR spectroscopy was confirmed as a rapid, precise approach for tracking molecular-level tissue alterations caused by toxic compounds.
HRW is proposed to scavenge reactive oxygen species generated by mercury exposure, thereby reducing oxidative DNA damage (as indicated by 8-Oxo-dG levels) and attenuating mercury-induced alterations in proteins, lipids, and polysaccharides at the molecular level.
Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).
See also:
https://h2-papers.org/en/papers/34718956