カドミウムストレス下のブラッシカ・カンペストリス幼苗における水素ガスの毒性軽減効果と一酸化窒素の関与
In Brassica campestris seedlings exposed to cadmium (Cd), hydrogen-rich water (HRW) pretreatment significantly improved growth parameters. Cd exposure elevated endogenous NO and H2 levels by 36% and 66%, respectively, with H2 accumulation preceding NO. Application of the NO scavenger PTIO or NO biosynthesis inhibitors (L-NAME and Gln) abolished the protective effects of HRW under Cd stress. HRW pretreatment promoted NO accumulation and upregulated both the activity and expression of nitrate reductase (NR). Concurrently, reactive oxygen species (ROS) were reduced, ascorbic acid (AsA) content increased, and activities of peroxidase (POD) and superoxide dismutase (SOD) were enhanced; these effects were reversed by NO inhibitors. Proteomic analysis identified 29 differentially expressed proteins, approximately 40% of which were associated with oxidation-reduction processes or antioxidant enzyme functions. The findings indicate that HRW-derived H2 stimulates NO biosynthesis via the NR pathway, and the combined action of H2 and NO strengthens antioxidant defenses against Cd toxicity.
HRW-derived H2 accumulation stimulates NO biosynthesis through the nitrate reductase (NR) pathway; H2 and NO then act cooperatively to upregulate antioxidant enzymes including SOD and POD, thereby reducing cadmium-induced oxidative damage in seedling roots.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
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https://h2-papers.org/en/papers/31071632