アルファルファにおける水素誘導カドミウム耐性の転写解析：硫黄および（ホモ）グルタチオン代謝の重要な役割
This study examined how hydrogen-rich water (HRW) confers cadmium (Cd) tolerance in alfalfa seedling roots using RNA-Seq transcriptome profiling. Under Cd and/or HRW conditions, 1,968 differentially expressed genes were identified, clustering into categories including glutathione (GSH) metabolism, oxidative stress response, and ABC transporter activity. RT-qPCR validation confirmed that HRW upregulated genes associated with sulfur and (homo)glutathione metabolism under Cd exposure. Pharmacological inhibition of glutathione synthesis and experiments with Arabidopsis thaliana cad2-1 mutants demonstrated the central contribution of glutathione to HRW-mediated Cd tolerance. Elevated (homo)glutathione and (homo)phytochelatin levels were consistent with reduced oxidative stress markers. Additionally, HRW appeared to lower bioavailable Cd in roots through ABC transporter-dependent secretion. Collectively, the findings indicate that molecular hydrogen modulates sulfur and glutathione metabolic gene networks, enhancing antioxidant capacity and Cd chelation as dual mechanisms underlying Cd tolerance.
Molecular hydrogen upregulates sulfur and (homo)glutathione metabolism genes in alfalfa roots, boosting antioxidant capacity and phytochelatin-mediated Cd chelation, while also promoting ABC transporter-dependent Cd secretion to reduce intracellular cadmium availability.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/32019510